Signal Transduction and Biotechnology Lab

Signal Transduction and Biotechnology Lab

Signal Transduction and Biotechnology Lab is mainly established for apoptosis related signal transduction studies. The lab is equipped with a Class II biological safety cabinet (BSC), a sealed bench-top centrifuge (used only for mammalian cells), two incubators and a water bath for cell culture work. In addition to these; a refrigerated bench-top centrifuge, an ELISA reader, a vacuum dryer, SDS-PAGE and western transfer apparatus, a balance, a magnetic stirrer/heater, freezers and refrigerators, and a vortex are available for DNA, RNA or protein studies. A chemical fume hood is located in the laboratory for working with hazardous chemicals. Cell culture stocks are kept in liquid nitrogen tank that is located in the cold room. Drugs and isolated proteins are kept in a -80 ºC freezer near the laboratory. Drug application, protein and RNA isolations, and all cell culture routines are conducted in the BSC. Western blotting, cellular transfections, real-time PCR, flow cytometry and microarray analyses are among the routines of the Signal Transduction and Biotechnology laboratory.

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